生长抑素在培养的大鼠松果体细胞中的表达。

Expression of somatostatin in rat pineal cells in culture.

作者信息

Mato E, Santisteban P, Viader M, Capellá G, Fornas O, Puig-Domingo M, Webb S M

机构信息

Department of Endocrinology, Hospital de Sant Pau, Universitat Autònoma de Barcelona, Spain.

出版信息

J Pineal Res. 1993 Aug;15(1):43-5. doi: 10.1111/j.1600-079x.1993.tb00508.x.

DOI:10.1111/j.1600-079x.1993.tb00508.x

PMID:7901362

Abstract

The expression of somatostatin mRNA was investigated in rat pineal cells after 1 week in culture, using reverse transcription of mRNA into cDNA and the polymerase chain reaction. The positive expression in cultured pineal cells demonstrates the capacity of this gland to synthesize somatostatin in denervated cells. Thus, apart from the neural origin of pineal somatostatin, which has been described in detail in the bovine species, a parenchymal source is demonstrated.

摘要

在培养1周后的大鼠松果体细胞中，采用将mRNA逆转录为cDNA及聚合酶链反应的方法，对生长抑素mRNA的表达进行了研究。培养的松果体细胞中的阳性表达表明该腺体在去神经支配的细胞中具有合成生长抑素的能力。因此，除了在牛物种中已详细描述的松果体生长抑素的神经起源外，还证实了一种实质来源。