Wegmann D R, Shehadeh N, Lafferty K J, Norbury-Glaser M, Gill R G, Daniel D
Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver 80262.
J Autoimmun. 1993 Oct;6(5):517-27. doi: 10.1006/jaut.1993.1043.
One impediment to detailed characterization of islet-specific T cells from the NOD mouse model of diabetes is the difficulty encountered in isolation of such cells. This report describes a method that allows routine isolation of relatively large numbers of T cells highly enriched for reactivity toward islet antigens. The method involves renal subcapsular transplantation of spontaneously diabetic NOD mice with NOD islets. These grafts are rapidly destroyed in a tissue-specific manner and this destruction is accompanied by lymphocytic infiltration. Here we demonstrate that the islet graft infiltrates are an excellent source of islet-specific T cells and that islet-specific T-cell lines and clones can be established from these cells. Islet-specific T-cell clones isolated from a T-cell line established from the islet graft infiltrates were capable of adoptive transfer of diabetes to NOD/LtSz-scid recipients.
从糖尿病NOD小鼠模型中详细鉴定胰岛特异性T细胞的一个障碍是分离此类细胞时遇到的困难。本报告描述了一种方法，该方法能够常规分离出大量对胰岛抗原有高反应性的T细胞。该方法包括将NOD胰岛移植到自发糖尿病的NOD小鼠的肾被膜下。这些移植物会以组织特异性方式迅速被破坏，并且这种破坏伴随着淋巴细胞浸润。在这里，我们证明胰岛移植物浸润细胞是胰岛特异性T细胞的极佳来源，并且可以从这些细胞中建立胰岛特异性T细胞系和克隆。从胰岛移植物浸润细胞建立的T细胞系中分离出的胰岛特异性T细胞克隆能够将糖尿病过继转移给NOD/LtSz-scid受体。
