He W, Kabelitz D
Institute of Immunology, University of Heidelberg, FRG.
Int Arch Allergy Immunol. 1993;102(4):321-6. doi: 10.1159/000236578.
Highly purified CD4-8- double-negative (DN) human thymocytes proliferated significantly more in response to interleukin (IL)-7 than to IL-2 in a dose-dependent fashion. Both IL-7 and IL-2 promoted the appearance of T-cell receptor (TCR) gamma delta-expressing cells in single cell suspension culture of DN thymocytes. While IL-7 exerted a vigorous growth-stimulating effect on TCR gamma delta-bearing DN thymocytes, IL-2 preferentially maintained the viability of gamma delta thymocytes without promoting their strong proliferation. In both instances, the major subset of gamma delta thymocytes expressed V delta 1 rather than V delta 2 as a variable delta-chain element on their surface. These results reveal differential effects of IL-7 and IL-2 on TCR gamma delta-bearing human thymocytes and underline the importance of both ILs in the development of DN thymocytes.
高度纯化的CD4 - 8 - 双阴性（DN）人胸腺细胞对白细胞介素（IL）-7的反应比IL - 2更显著，呈剂量依赖性增殖。在DN胸腺细胞的单细胞悬浮培养中，IL - 7和IL - 2均促进了表达T细胞受体（TCR）γδ的细胞的出现。虽然IL - 7对携带TCRγδ的DN胸腺细胞具有强烈的生长刺激作用，但IL - 2优先维持γδ胸腺细胞的活力，而不促进其强烈增殖。在这两种情况下，γδ胸腺细胞的主要亚群在其表面表达Vδ1而非Vδ2作为可变δ链元件。这些结果揭示了IL - 7和IL - 2对携带TCRγδ的人胸腺细胞的不同作用，并强调了这两种白细胞介素在DN胸腺细胞发育中的重要性。
