牛视网膜杆状细胞中存在一种含对钙敏感的p26的复合物。

The presence of a calcium-sensitive p26-containing complex in bovine retina rod cells.

作者信息

Gorodovikova E N, Philippov P P

机构信息

Department of Enzymology, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.

出版信息

FEBS Lett. 1993 Dec 6;335(2):277-9. doi: 10.1016/0014-5793(93)80746-h.

DOI:10.1016/0014-5793(93)80746-h

PMID:7902818

Abstract

A protein with apparent M(r) 26 kDa (p26 [(1991) Biokimya 56, 225-228] or recoverin [(1991) Science 251, 915-918]) was suggested to activate GC in a calcium dependent manner in bovine retina rod cells [(1991) Science 251, 915-918; (1991) EMBO J, 10, 793-798]. However, according to our present data homogeneous p26 preparations do not activate the enzyme. At the same time we have revealed a complex of p26 with an unidentified protein, presumably RK, in bovine ROS. Calcium favours formation of the complex whereas EGTA addition (which corresponds to a low free Ca2+ concentration) leads to its dissociation.

摘要

一种表观分子量为26 kDa的蛋白质（p26 [(1991) 《生物化学》56, 225 - 228] 或恢复蛋白 [(1991) 《科学》251, 915 - 918]）被认为在牛视网膜杆状细胞中以钙依赖的方式激活鸟苷酸环化酶 [(1991) 《科学》251, 915 - 918; (1991) 《欧洲分子生物学组织杂志》, 10, 793 - 798]。然而，根据我们目前的数据，纯化的p26制剂并不能激活该酶。同时，我们在牛视网膜外段中发现了p26与一种未鉴定的蛋白质（可能是RK）形成的复合物。钙有利于复合物的形成，而添加乙二醇双（2 - 氨基乙基醚）四乙酸（对应于低游离Ca2+浓度）会导致其解离。