Botrè F, Botrè C, Lorenti G, Mazzei F, Porcelli F, Scibona G
Istituto di Merceologia, Rome, Italy.
J Pharm Biomed Anal. 1993 Aug;11(8):679-86. doi: 10.1016/0731-7085(93)80174-y.
An amperometric biosensor for the direct determination of L-glutamate was developed by chemical bonding of L-glutamate oxidase (GAO) on a carboxylic Nylon membrane with polyazetidine prepolymer (PAP), and using a hydrogen peroxide electrode as indicating sensor. The biosensor is specific for L-glutamate and the peculiar analytical properties (linearity range, reproducibility, accuracy) were experimentally determined. Furthermore, the same basic biosensor was also modified to be used and characterized for the direct determination of L-glutamine. This L-glutamine biosensor was obtained by coimmobilizing, on two separate membranes, glutamic acid oxidase and glutaminase (GMN) on the same biosensor. The two sensors were then used for the determination of glutamate and L-glutamine contained in pharmaceutical formulations and the results were compared with those obtained by other analytical methods.
通过将L-谷氨酸氧化酶（GAO）与聚氮杂环丁烷预聚物（PAP）化学键合在羧基尼龙膜上，并使用过氧化氢电极作为指示传感器，开发了一种用于直接测定L-谷氨酸的电流型生物传感器。该生物传感器对L-谷氨酸具有特异性，并通过实验确定了其独特的分析特性（线性范围、重现性、准确性）。此外，对相同的基本生物传感器进行了修饰，使其可用于直接测定L-谷氨酰胺并对其进行表征。这种L-谷氨酰胺生物传感器是通过将谷氨酸氧化酶和谷氨酰胺酶（GMN）共固定在同一生物传感器的两个单独膜上而获得的。然后将这两种传感器用于测定药物制剂中所含的谷氨酸和L-谷氨酰胺，并将结果与其他分析方法获得的结果进行比较。
