Song L, Adler W H, Chung S, Kim Y H, TenBrook J, Collins G D, Nagel J E
Clinical Immunology Section, National Institute on Aging, NIH, Baltimore, MD 21224.
Immunology. 1993 Oct;80(2):222-8.
The tyrosine phosphorylated protein(s) responsible for the signalling for interleukin-2 (IL-2) production has not been clearly defined. In this study, the relationship between IL-2 production and the protein tyrosine phosphorylation pattern of human Jurkat T cells was investigated using phosphotyrosine immunoblotting analysis. With anti-CD3 or anti-CD2 activation the cells showed only a low (anti-CD3) or a moderate (anti-CD2) level of tyrosine phosphorylation of a 42,000 MW external signal-regulated kinase (ERK), which was accompanied by undetectable (anti-CD3) or low level (anti-CD2) IL-2 production. In the presence of phorbol myristate acetate (PMA), large amounts of IL-2 were induced by both anti-CD3 and anti-CD2 stimulation, which was accompanied by strong concurrent tyrosine phosphorylation of the 42,000 MW ERK and a 100,000 MW protein. PMA alone, which induced high levels of tyrosine phosphorylation of the ERK protein, neither induced any detectable IL-2 nor increased the level of tyrosine phosphorylation of the 100,000 MW protein. These observations suggest that concurrent tyrosine phosphorylation of the 42,000 MW ERK and a 100,000 MW protein may be required for IL-2 production.
负责白细胞介素-2（IL-2）产生信号传导的酪氨酸磷酸化蛋白尚未明确界定。在本研究中，利用磷酸酪氨酸免疫印迹分析研究了IL-2产生与人Jurkat T细胞蛋白酪氨酸磷酸化模式之间的关系。用抗CD3或抗CD2激活后，细胞显示出42,000道尔顿的细胞外信号调节激酶（ERK）只有低水平（抗CD3）或中等水平（抗CD2）的酪氨酸磷酸化，同时伴随无法检测到的（抗CD3）或低水平（抗CD2）的IL-2产生。在佛波醇肉豆蔻酸酯乙酸酯（PMA）存在的情况下，抗CD3和抗CD2刺激均诱导大量IL-2产生，同时伴随42,000道尔顿的ERK和100,000道尔顿蛋白强烈的酪氨酸磷酸化。单独的PMA可诱导ERK蛋白高水平的酪氨酸磷酸化，但既不诱导任何可检测到的IL-2，也不增加100,000道尔顿蛋白的酪氨酸磷酸化水平。这些观察结果表明，42,000道尔顿的ERK和100,000道尔顿蛋白同时发生酪氨酸磷酸化可能是IL-2产生所必需的。
