Lamont R J, Bevan C A, Gil S, Persson R E, Rosan B
Department of Oral Biology, School of Dentistry, University of Washington, Seattle.
Oral Microbiol Immunol. 1993 Oct;8(5):272-6. doi: 10.1111/j.1399-302x.1993.tb00573.x.
Adherence of Porphyromonas gingivalis to early plaque bacteria, such as Streptococcus gordonii, is considered an important colonization mechanism. The molecules that mediate this interspecies binding have not been determined. Fimbriae were prepared from P. gingivalis 33277 by mild agitation, ammonium sulfate precipitation and DEAE-Sepharose chromatography. In a nitrocellulose blot adherence assay, purified fimbriae inhibited S. gordonii G9B-P. gingivalis 33277 binding by up to 54%. In addition, fimbriae bound to S. gordonii cells in a dot-blot assay. Incubation of fimbriae with S. gordonii cells followed by washing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), electroblotting and probing with P. gingivalis antibodies also revealed that the fimbriae bind to S. gordonii. In contrast, S. gordonii did not interact with fimbriae that were first subjected to SDS-PAGE and electroblotting or deposited on a nitrocellulose membrane, suggesting that conformational determinants of the fimbriae may be important in binding. The results indicate that binding between P. gingivalis and S. gordonii is mediated, at least in part, by the porphyromonads' fimbriae.
牙龈卟啉单胞菌对早期菌斑细菌（如戈登链球菌）的黏附被认为是一种重要的定植机制。介导这种种间结合的分子尚未确定。通过温和搅拌、硫酸铵沉淀和DEAE-琼脂糖层析从牙龈卟啉单胞菌33277制备菌毛。在硝酸纤维素膜印迹黏附试验中，纯化的菌毛可使戈登链球菌G9B与牙龈卟啉单胞菌33277的结合抑制达54%。此外，在斑点印迹试验中菌毛可与戈登链球菌细胞结合。将菌毛与戈登链球菌细胞孵育，随后洗涤、进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、电印迹并用牙龈卟啉单胞菌抗体进行检测，结果也显示菌毛可与戈登链球菌结合。相反，戈登链球菌不与先经SDS-PAGE和电印迹处理或沉积在硝酸纤维素膜上的菌毛相互作用，这表明菌毛的构象决定簇在结合中可能很重要。结果表明，牙龈卟啉单胞菌与戈登链球菌之间的结合至少部分是由卟啉单胞菌的菌毛介导的。
