Bauer M B, Simmons M L, Murphy S, Gebhart G F
Department of Pharmacology, College of Medicine, University of Iowa, Iowa City 52242.
J Neurosci Res. 1993 Oct 15;36(3):280-9. doi: 10.1002/jnr.490360306.
Dorsal root ganglion (DRG) neurons express receptors for bradykinin and capsaicin, both algesic substances. Administration of bradykinin or capsaicin to neurons cultured from embryonic rat DRG stimulated the production of cyclic GMP but did not affect the production of cyclic GMP in nonneuronal DRG cultures. Bradykinin-evoked cyclic GMP production was mediated by B2 receptors and was unaltered by indomethacin. Both bradykinin- and capsaicin-stimulated cyclic GMP production required Ca2+ and was inhibited by methylene blue. Furthermore, methylene blue attenuated basal cyclic GMP production in DRG neurons, suggesting tonic cyclic GMP production in these cells. L-NG-monomethyl arginine inhibited both bradykinin- and capsaicin-stimulated cyclic GMP production as well as basal cyclic GMP production. These findings suggest the involvement of a nitrosyl compound in bradykinin- and capsaicin-stimulated cyclic GMP production and in tonic cyclic GMP production in DRG neurons.
背根神经节（DRG）神经元表达缓激肽和辣椒素的受体，这两种都是致痛物质。将缓激肽或辣椒素施用于从胚胎大鼠DRG培养的神经元，可刺激环鸟苷酸（cGMP）的产生，但不影响非神经元DRG培养物中环鸟苷酸的产生。缓激肽诱发的环鸟苷酸产生由B2受体介导，且不受吲哚美辛影响。缓激肽和辣椒素刺激的环鸟苷酸产生均需要Ca2+，并被亚甲蓝抑制。此外，亚甲蓝减弱了DRG神经元中的基础环鸟苷酸产生，表明这些细胞中存在持续性环鸟苷酸产生。L-NG-单甲基精氨酸抑制缓激肽和辣椒素刺激的环鸟苷酸产生以及基础环鸟苷酸产生。这些发现表明亚硝基化合物参与了缓激肽和辣椒素刺激的环鸟苷酸产生以及DRG神经元中的持续性环鸟苷酸产生。
