芦笋中收获诱导型天冬酰胺合成酶cDNA克隆的分离与鉴定
Isolation and characterization of a cDNA clone for a harvest-induced asparagine synthetase from Asparagus officinalis L.
作者信息
Davies K M, King G A
机构信息
New Zealand Institute for Crop & Food Research Limited, Levin Research Centre.
出版信息
Plant Physiol. 1993 Aug;102(4):1337-40. doi: 10.1104/pp.102.4.1337.
Abstract
A full-length cDNA clone (pTIP27) encoding asparagine synthetase (AS; EC 6.3.5.4) was isolated from a cDNA library prepared from the tip section (apex to 30 mm) of Asparagus officinalis L. spears. The cDNA clone encodes an mRNA of 1978 bp, giving a derived protein of 66.5 kD molecular mass. The derived amino acid sequence is 81% homologous to AS from Pisum sativum. Only low levels of transcript for AS could be detected in growing spears, roots, or ferns. However, AS mRNA levels began to increase in the tips of harvested spears after 2 h at 20 degrees C, and in the other sections of the spear after 4 h, suggesting that all sections of the spear were responding to the same postharvest signal. The results are discussed in relation to metabolic changes occurring in harvested spears.
摘要
从芦笋嫩茎顶端部分（从顶端到30毫米处）构建的cDNA文库中分离出一个编码天冬酰胺合成酶（AS；EC 6.3.5.4）的全长cDNA克隆（pTIP27）。该cDNA克隆编码一个1978 bp的mRNA，推导的蛋白质分子量为66.5 kD。推导的氨基酸序列与豌豆中的AS有81%的同源性。在生长的嫩茎、根或叶状枝中只能检测到低水平的AS转录本。然而，在20℃下放置2小时后，收获嫩茎顶端的AS mRNA水平开始增加，4小时后在嫩茎的其他部分也开始增加，这表明嫩茎的所有部分都在对相同的采后信号做出反应。结合采后嫩茎中发生的代谢变化对结果进行了讨论。
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