Juhlin L, Shroot B
Department of Dermatology, University of Uppsala, Sweden.
Acta Derm Venereol. 1993 Aug;73(4):273-5. doi: 10.2340/0001555573273275.
The area of UV erythema produced by a small beam head was found to increase with increasing doses. The aim was to investigate whether measurement of the area could be a more useful indicator of UV-induced damage than classic visual grading. Topical pretreatment with all-trans retinoic acid (tretinoin) and betamethasone valerate was used to test the applicability of the method in pharmacological studies. We used a round outlet head (5 mm2) connected by optical fibre to a monochromatic irradiator, and doses ranging from 0.05 to 0.2 Joule of 300 mm UV light were applied to the skin of 6 healthy subjects. Erythemal area was calculated by the measurement of two diameters, and intensity was graded visually (0-6 scale). The area of the erythema correlated with the increase in intensity up to score 6. Area measurement was less subject to intra-investigators' variability than the intensity score. By multiplying intensity by area, a good indicator of UV-induced reactivity was obtained. Pretreatment with betamethasone valerate decreased the area of erythema, as did tretinoin 12 h after irradiation. Thus, area measurement of erythema is a useful adjunct to visual grading of UV-induced skin reactions.
研究发现，小光斑头产生的紫外线红斑面积会随着剂量增加而增大。目的是探究红斑面积测量是否比传统的视觉分级更能有效指示紫外线引起的损伤。采用全反式维甲酸（维甲酸）和戊酸倍他米松进行局部预处理，以测试该方法在药理学研究中的适用性。我们使用一个通过光纤连接到单色辐照器的圆形出口头（5平方毫米），对6名健康受试者的皮肤施加0.05至0.2焦耳的300纳米紫外线，剂量范围从0.05到0.2焦耳。通过测量两个直径来计算红斑面积，并对红斑强度进行视觉分级（0 - 6级）。红斑面积与强度增加相关，直至6分。与强度评分相比，面积测量受研究者内部变异性的影响较小。通过将强度乘以面积，得到了一个很好的紫外线诱导反应性指标。戊酸倍他米松预处理可减少红斑面积，照射12小时后维甲酸预处理也有同样效果。因此，红斑面积测量是紫外线诱导皮肤反应视觉分级的有用辅助手段。
