Vodkin M H, McLaughlin G L, Day J F, Shope R E, Novak R J
Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana.
Am J Trop Med Hyg. 1993 Dec;49(6):772-6. doi: 10.4269/ajtmh.1993.49.772.
Eastern equine encephalomyelitis virus (EEEV) has been a low-frequency, but serious human and veterinary health problem. Increased frequency of this mosquito-borne virus is anticipated as wetlands are maintained and re-established. Control of EEEV has depended on mosquito abatement in response to increasing frequency of EEEV in the environment. A coupled reverse transcription/polymerase chain reaction assay was designed to rapidly, sensitively, and specifically detect EEEV RNA. The assay successfully detected the viral RNA in a single-blind study of a set of field samples composed of either pooled mosquitoes or bird tissue. These results suggest that it would be practical to use this assay for deciding when and where to implement mosquito abatement.
东部马脑脊髓炎病毒（EEEV）一直是一个低频但严重的人类和兽医健康问题。随着湿地的维持和重建，预计这种蚊媒病毒的发病率会增加。EEEV的控制依赖于随着环境中EEEV发病率增加而进行的蚊虫防治。设计了一种逆转录/聚合酶链反应联合检测方法，用于快速、灵敏且特异性地检测EEEV RNA。该检测方法在一组由混合蚊虫或鸟类组织组成的野外样本的单盲研究中成功检测到了病毒RNA。这些结果表明，使用该检测方法来决定何时何地进行蚊虫防治是可行的。
