DNA扩增技术在兽医诊断中的应用。

Applications of DNA amplification techniques in veterinary diagnostics.

作者信息

Pfeffer M, Wiedmann M, Batt C A

机构信息

Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig-Maximilians University, Munich, Germany.

出版信息

Vet Res Commun. 1995;19(5):375-407. doi: 10.1007/BF01839319.

DOI:10.1007/BF01839319

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7089009/

Abstract

An overview of the principles of the polymerase chain reaction, ligase chain reaction, self-sustained sequence replication and Q beta replicase is given. The application of these methods for the diagnosis of veterinary infectious and hereditary diseases as well as for other diagnostic purposes is discussed and comprehensive tables of reported assays are provided. Specific areas where these DNA-based amplification methods provide substantial advantages over traditional approaches are also highlighted. With regard to PCR-based assays for the detection of viral pathogens, this article is an update of a previous review by Belák and Ballagi-Pordány (1993).

摘要

本文概述了聚合酶链反应、连接酶链反应、自我维持序列复制和Qβ复制酶的原理。讨论了这些方法在兽医传染病和遗传性疾病诊断以及其他诊断目的中的应用，并提供了已报道检测方法的综合表格。还强调了这些基于DNA的扩增方法相对于传统方法具有显著优势的特定领域。关于基于PCR的病毒病原体检测方法，本文是对Belák和Ballagi-Pordány（1993年）之前一篇综述的更新。

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