Curcuruto O, Hamdan M
Glaxo Research Laboratories, Verona, Italy.
Rapid Commun Mass Spectrom. 1993 Nov;7(11):989-93. doi: 10.1002/rcm.1290071105.
A number of protonated neuropetides formed by 10 keV Cs+ ion bombardment are mass selected and their collision-induced dissociations (CID) with helium, argon, xenon and isobutane are monitored using a collision energy of 200 eV. Data obtained by fixing the collision gas and using translational energies in the range 50-250 eV are also presented. The present data imply that optimization of the CID spectra presented can be achieved through a judicious choice of both the target gas and the translational energy of the mass-selected ion. The combined effect of the two experimental parameters on the relative abundances of the major sequence ions and on the loss of side-chain moieties is discussed.
通过10 keV Cs⁺离子轰击形成的多种质子化神经肽被进行质量选择，并使用200 eV的碰撞能量监测它们与氦气、氩气、氙气和异丁烷的碰撞诱导解离（CID）。还展示了通过固定碰撞气体并使用50 - 250 eV范围内的平动能所获得的数据。目前的数据表明，通过明智地选择目标气体和质量选择离子的平动能，可以实现所呈现的CID光谱的优化。讨论了这两个实验参数对主要序列离子的相对丰度以及侧链部分损失的综合影响。
