Tunturi A R
Physiol Chem Phys. 1976;8(2):107-14.
A new method has been developed for silver impregnation of neurons and myelinated axons in the cerebral cortex of the adult dog. By this method principal types of neurons including pyramidal, stellate, fusiform, horizontal neurons of Layer I, and special auditory neurons are colored black with a clear background, while myelinated fibers in the same piece of tissue are colored yellow. The yellow hue appears limited to the myelin sheath. Meyelinated axons of all sizes in the molecular layer, radial bundles, and deep tangential strata accept the stain. Comparison with data from other experiments indicates that osmium tetroxide inhibits the yellow staining of the myelinated axons, and that formaldehyde promotes such staining in conjunction with ammonium ions and sucrose.
已开发出一种用于成年犬大脑皮质神经元和有髓轴突银浸染的新方法。通过这种方法，包括锥体细胞、星状细胞、梭形细胞、I层水平神经元和特殊听觉神经元在内的主要神经元类型均被染成黑色，背景清晰，而同一组织中的有髓纤维则被染成黄色。黄色调似乎仅限于髓鞘。分子层、放射束和深切线层中各种大小的有髓轴突均可接受染色。与其他实验数据的比较表明，四氧化锇会抑制有髓轴突的黄色染色，而甲醛与铵离子和蔗糖一起可促进这种染色。
