Voeller H J, Sugars L Y, Pretlow T, Gelmann E P
Division of Medical Oncology, Lombardi Cancer Research Center, Washington, D.C.
J Urol. 1994 Feb;151(2):492-5. doi: 10.1016/s0022-5347(17)35000-0.
Eighty-five prostate cancer specimens from prostate resections were analyzed for the presence of p53 gene mutations by immunohistochemical staining for P53 protein. DNA from thirty-four of these samples was also analyzed for mutations in exons 5-8 by single-strand chain polymorphism (SSCP) analysis. One sample had P53 staining by immunohistochemistry, one sample was positive for a p53 mutation by SSCP, and one sample was positive by both techniques. Mutations in the two samples that were positive by SSCP were confirmed by nucleotide sequencing. In a separate study of ten lymph nodes that contained prostate cancer metastases, one had detectable P53 protein by immunohistochemical staining. Therefore, p53 mutations appear to be low frequency events in primary prostate cancer.
通过对P53蛋白进行免疫组织化学染色，分析了85例前列腺切除术后的前列腺癌标本中p53基因突变的情况。还通过单链构象多态性（SSCP）分析对其中34个样本的DNA进行了外显子5-8的突变分析。一个样本通过免疫组织化学检测到P53染色，一个样本通过SSCP检测p53突变呈阳性，还有一个样本两种技术检测均呈阳性。通过SSCP检测呈阳性的两个样本中的突变经核苷酸测序得以证实。在另一项对10个含有前列腺癌转移灶的淋巴结的研究中，有一个通过免疫组织化学染色检测到可检测到的P53蛋白。因此，p53突变在原发性前列腺癌中似乎是低频事件。
