Liu C P, Globerson A, Auerbach R
Center for Developmental Biology, University of Wisconsin, Madison.
Thymus. 1993 Jun;21(4):221-33.
We have previously established conditions under which murine yolk sac cells can be maintained in vitro in the absence of thymus, and we reported that a spontaneously transformed Thy1.2+ yolk sac cell line was obtained after long term culture in vitro. We now provide further phenotypic characterization of a clone, YSA1, of this Thy1.2+ cell line showing that it expresses Thy1.2, CD3/TCR V beta 8, IL-2 receptor (IL2R), and heat stable antigen (HSAg), but does not express CD4/CD8 or TCR gamma delta. The YSA1 clone is an immature cell as indicated by the expression of IL2R and HSAg and by nonproduction of IL-2 upon stimulation by anti-CD3 antibody. The data show that yolk sac stem cells can differentiate into CD3/TCR expressing cells in the absence of thymus, but do not progress in vitro paralleling observations made on freshly-isolated yolk sac stem cells.
我们之前已经建立了在无胸腺条件下体外培养小鼠卵黄囊细胞的条件，并且我们报道过在体外长期培养后获得了一株自发转化的Thy1.2⁺卵黄囊细胞系。现在我们对该Thy1.2⁺细胞系的一个克隆YSA1进行了进一步的表型特征分析，结果显示它表达Thy1.2、CD3/TCR Vβ8、白细胞介素-2受体（IL2R）和热稳定抗原（HSAg），但不表达CD4/CD8或TCRγδ。YSA1克隆是一种未成熟细胞，这可通过IL2R和HSAg的表达以及抗CD3抗体刺激后不产生白细胞介素-2来表明。数据表明，卵黄囊干细胞在无胸腺的情况下可分化为表达CD3/TCR的细胞，但在体外不会像对新鲜分离的卵黄囊干细胞所做的观察那样继续分化。
