果蝇黑腹 IMP 脱氢酶编码 cDNA 的克隆与序列分析
Cloning and sequence analysis of a Drosophila melanogaster cDNA encoding IMP dehydrogenase.
作者信息
Sifri C D, Wilson K, Smolik S, Forte M, Ullman B
机构信息
Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland 97201.
出版信息
Biochim Biophys Acta. 1994 Jan 18;1217(1):103-6.
A cDNA encoding the entire Drosophila melanogaster IMP dehydrogenase (IMPDH) protein was isolated and sequenced. Translation of the impdh cDNA nucleotide sequence indicated that the Drosophila IMPDH exhibited 48% and 65% amino acid identity to the Leishmania donovani and human isoform II counterparts, respectively. Northern analysis revealed that expression of the 2.4 kb impdh transcript was equivalent in adult Drosophila head and body and that impdh expression was developmentally regulated. In situ hybridization of the cloned impdh cDNA probe to Drosophila salivary gland chromosomes indicated that the impdh gene is located at position 9E1-4 on the X chromosome.
分离并测序了编码完整黑腹果蝇肌苷酸脱氢酶（IMPDH）蛋白的cDNA。对impdh cDNA核苷酸序列的翻译表明，果蝇IMPDH与杜氏利什曼原虫和人类同工型II对应物的氨基酸同一性分别为48%和65%。Northern分析显示，2.4 kb impdh转录本在成年果蝇头部和身体中的表达相当，并且impdh的表达受到发育调控。将克隆的impdh cDNA探针与果蝇唾液腺染色体进行原位杂交表明，impdh基因位于X染色体的9E1-4位置。
Zotero 插件