Hernandez L, Tucci S, Guzman N, Paez X
Laboratory of Behavioral Physiology, Medical School, Los Andes University, Merida, Venezuela.
J Chromatogr A. 1993 Oct 22;652(2):393-8. doi: 10.1016/0021-9673(93)83258-T.
Glutamic acid, an excitatory neurotransmitter, was monitored in vivo in the corpus striatum of freely moving rats by brain microdialysis and capillary electrophoresis with laser-induced fluorescence detection. A procedure to derivatize glutamate in complex matrices was developed. Capillary electrophoresis in 12 microns I.D. capillaries was performed to determine glutamate with a migration time of 195 s. Laser-induced fluorescence detection with 488-nm radiation from an argon ion laser and with colinear geometry was used. An injection of haloperidol decreased the concentration of glutamic acid in the dialysates. These experiments support the hypothesis that dopamine receptor blockade decreases glutamate release. The potential of these techniques for the study of chemicals in biomedical experiments is discussed.
谷氨酸是一种兴奋性神经递质，通过脑微透析和激光诱导荧光检测的毛细管电泳技术，在自由活动大鼠的纹状体中对其进行了体内监测。开发了一种在复杂基质中衍生化谷氨酸的方法。使用内径为12微米的毛细管进行毛细管电泳，以测定谷氨酸，其迁移时间为195秒。采用氩离子激光器发射的488纳米辐射和共线几何结构进行激光诱导荧光检测。注射氟哌啶醇可降低透析液中谷氨酸的浓度。这些实验支持了多巴胺受体阻断会减少谷氨酸释放这一假说。讨论了这些技术在生物医学实验中研究化学物质的潜力。
