Bingen E
Laboratoire de Microbiologie, Hôpital Robert Debré, Paris, France.
Pathol Biol (Paris). 1993 Oct;41(8):716-23.
Nosocomial infection cause severe morbidity and mortality in pediatric patients. To find the cause of an infectious disease outbreak, epidemiologists need to determine early on whether a single strain of microorganism is responsible for the majority of cases. Phenotypic characteristics have been widely used in epidemiologic studies. However with most systems poor reproducibility, poor sensitivity have been reported and they do not reliably show enough strain-to-strain variation to be sufficiently discriminative. Molecular approaches like the analysis of the restriction fragment length polymorphism of the total DNA and of the rDNA regions and pulsed-field gel electrophoresis typing have now been applied with success to a large number of bacterial species associated with epidemics.
医院感染可导致儿科患者出现严重的发病率和死亡率。为了找出传染病暴发的原因，流行病学家需要尽早确定是否单一菌株的微生物导致了大多数病例。表型特征已广泛应用于流行病学研究。然而，据报道，大多数系统的重现性差、灵敏度低，并且它们不能可靠地显示出足够的菌株间差异以进行充分区分。像分析总DNA和rDNA区域的限制性片段长度多态性以及脉冲场凝胶电泳分型等分子方法现已成功应用于大量与流行病相关的细菌种类。
