IgA肾病患者肾活检样本及血清中的副流感嗜血杆菌抗原和抗体。

Haemophilus parainfluenzae antigen and antibody in renal biopsy samples and serum of patients with IgA nephropathy.

作者信息

Suzuki S, Nakatomi Y, Sato H, Tsukada H, Arakawa M

机构信息

Department of Medicine (II), Niigata University School of Medicine, Japan.

出版信息

Lancet. 1994 Jan 1;343(8888):12-6. doi: 10.1016/s0140-6736(94)90875-3.

DOI:10.1016/s0140-6736(94)90875-3

PMID:7905040

Abstract

IgA nephropathy may be associated with colonisation with Haemophilus parainfluenzae. In patients with glomerular diseases, we examined renal-biopsy specimens for presence of bacterial antigen by immunofluorescence microscopy with rabbit antiserum against H parainfluenzae, and by enzyme-linked immunosorbent assay looked for IgA antibody against H parainfluenzae in patient sera. The rabbit antiserum recognised by immunoblotting four components of H parainfluenzae outer membranes (OMHP) of molecular weights 19.5, 30, 33, and 40.5 kDa. All 44 patients with IgA nephropathy and 2 of 39 patients with other glomerular diseases showed mesangial deposition of OMHP antigens (p < 0.001). Patients with IgA nephropathy had significantly more IgA antibody against H parainfluenzae than did patients with other glomerular diseases. IgA antibody in the sera of patients with IgA nephropathy recognised by immunoblotting the same four components of OMHP as recognised by rabbit antiserum. Glomerular deposition of OMHP antigens and the presence of IgA antibody against OMHP in patients with IgA nephropathy suggest that H parainfluenzae has a role in the aetiology of this disease.

摘要

IgA肾病可能与副流感嗜血杆菌定植有关。在肾小球疾病患者中，我们通过用抗副流感嗜血杆菌兔抗血清进行免疫荧光显微镜检查，检测肾活检标本中细菌抗原的存在，并通过酶联免疫吸附测定法在患者血清中寻找抗副流感嗜血杆菌的IgA抗体。兔抗血清通过免疫印迹法识别副流感嗜血杆菌外膜（OMHP）的四种分子量分别为19.5、30、33和40.5 kDa的成分。所有44例IgA肾病患者和39例其他肾小球疾病患者中的2例均显示OMHP抗原的系膜沉积（p<0.001）。IgA肾病患者抗副流感嗜血杆菌的IgA抗体明显多于其他肾小球疾病患者。IgA肾病患者血清中的IgA抗体通过免疫印迹法识别与兔抗血清识别的相同的OMHP四种成分。IgA肾病患者中OMHP抗原的肾小球沉积以及抗OMHP的IgA抗体的存在表明副流感嗜血杆菌在该疾病的病因学中起作用。