齐多夫定对人类淋巴细胞亚群微核的诱导作用。

AZT-induction of micronuclei in human lymphocyte subpopulations.

作者信息

Stern M, Cid M G, Larripa I, Slavutsky I

机构信息

Departamento de Citogenética, Instituto de Investigaciones Hematológicas Mariano R. Castex, Academia Nacional de Medicina, Buenos Aires, Argentina.

出版信息

Toxicol Lett. 1994 Feb 1;70(2):235-42. doi: 10.1016/0378-4274(94)90167-8.

DOI:10.1016/0378-4274(94)90167-8

PMID:7905210

Abstract

Micronucleus (MN) induction by azidothymidine (AZT) in B and T lymphocytes was analyzed by a recently developed MAC (morphology-antibody-chromosome) method which allows the immunologic identification of different cell lineages. An increased frequency of MN in AZT-treated cultures compared with controls was observed. CD4 cells were found to be more sensitive to AZT damage. AZT-treated cultures showed a significant decrease in the proportion of CD4 interphasic cells. Furthermore, higher MN frequencies in isolated lymphocytes than in whole blood in both control and AZT-treated cultures were observed.

摘要

通过最近开发的MAC（形态学-抗体-染色体）方法分析了齐多夫定（AZT）在B和T淋巴细胞中诱导微核（MN）的情况，该方法能够对不同细胞谱系进行免疫学鉴定。与对照组相比，观察到AZT处理的培养物中MN频率增加。发现CD4细胞对AZT损伤更敏感。AZT处理的培养物中CD4间期细胞的比例显著降低。此外，在对照和AZT处理的培养物中，均观察到分离淋巴细胞中的MN频率高于全血中的MN频率。