抗中性粒细胞胞浆抗体（ANCA）可增强中性粒细胞对培养的人内皮细胞的黏附。

Anti-neutrophil cytoplasm antibodies (ANCA) increase neutrophil adhesion to cultured human endothelium.

作者信息

Keogan M T, Rifkin I, Ronda N, Lockwood C M, Brown D L

机构信息

Dept. of Clinical Immunology, Addenbrooke's Hospital, Cambridge.

出版信息

Adv Exp Med Biol. 1993;336:115-9. doi: 10.1007/978-1-4757-9182-2_19.

DOI:10.1007/978-1-4757-9182-2_19

PMID:7905228

Abstract

ANCA have been demonstrated to activate normal neutrophils in vitro. This study demonstrates that ANCA positive IgG and Fab2 fragments increase the adhesion of normal neutrophils to cultured human umbilical vein endothelium (HUVEC), and that preincubation of the endothelial monolayers with tumour necrosis factor (TNF) to activate the endothelium enhances this effect. Preincubation of neutrophils with anti-CD18 monoclonal antibodies inhibited adhesion, suggesting that the observed effect was integrin mediated.

摘要

抗中性粒细胞胞浆抗体（ANCA）已被证实在体外可激活正常中性粒细胞。本研究表明，ANCA阳性IgG和Fab2片段可增加正常中性粒细胞与培养的人脐静脉内皮细胞（HUVEC）的黏附，并且用肿瘤坏死因子（TNF）预孵育内皮单层细胞以激活内皮可增强这种效应。用抗CD18单克隆抗体预孵育中性粒细胞可抑制黏附，提示观察到的效应是由整合素介导的。