Fujii H, Tanigawa N, Muraoka R, Shimomatsuya T, Tanaka T
Second Department of Surgery, Fukui Medical School, Japan.
Anticancer Res. 1993 Nov-Dec;13(6A):2171-5.
P-glycoprotein (P-gp) detection by FCM (flow cytometry) was correlated with P-gp detection by immunohistochemical staining or Western blotting in K562 cell lines variably resistant to doxorubicin (DOX). A strong correlation was noted between the flow cytometric data and the degree of resistance assessed by thymidine incorporation assay (TIA). Of the human carcinomas tested, 37.5% were P-gp positive by flow cytometry. The flow cytometry data correlated strongly with the immunohistochemical staining (p < 0.001). Thirteen of the 15 P-gp positive tumors were resistant to DOX and vincristine (VCR) on TIA. Thus, P-gp detection by FCM correlates strongly with tumor sensitivity to DOX and VCR (p < 0.001).
在对阿霉素（DOX）具有不同耐药性的K562细胞系中，通过流式细胞术（FCM）检测P-糖蛋白（P-gp）与通过免疫组织化学染色或蛋白质印迹法检测P-gp相关。流式细胞术数据与通过胸腺嘧啶核苷掺入试验（TIA）评估的耐药程度之间存在强相关性。在所测试的人类癌组织中，37.5%通过流式细胞术检测为P-gp阳性。流式细胞术数据与免疫组织化学染色密切相关（p<0.001）。在TIA中，15个P-gp阳性肿瘤中有13个对DOX和长春新碱（VCR）耐药。因此，通过FCM检测P-gp与肿瘤对DOX和VCR的敏感性密切相关（p<0.001）。
