Gulati S, Lemoli R, Fraig M
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York 10021.
Stem Cells. 1993 Oct;11 Suppl 3:14-9. doi: 10.1002/stem.5530110906.
Allogeneic bone marrow transplantation (BMT) is a useful approach for treating patients with chronic myelogenous leukemia (CML). In patients who lack a suitable donor, various approaches to utilize the patient's own hematopoietic stem cells obtained from bone marrow and/or peripheral blood have been developed. Preferential recovery of normal human hematopoietic progenitors over CML clone in long-term bone marrow culture (LTBMC) has been observed for a long time. LTBMC initiated in tissue culture (TC) flasks to that in "Lifecell" bags, which are gas-permeable plastic bags in which feeder-layer cells cannot adhere, has been investigated for several years. In our laboratory, cells were incubated in the presence of various growth factors to develop improved methods for stem cell expansion. Sustained hematopoietic stem cell growth in the absence of a feeder layer in plastic gas-permeable bags has been observed. Growth of marrow from patients with CML is being investigated extensively. Combining effective drugs to decrease the Philadelphia (Ph) clone prior to bone marrow harvest and the use of cultured bone marrow may provide a useful method for treating patients with CML. Results of various international studies are also discussed below.
异基因骨髓移植（BMT）是治疗慢性粒细胞白血病（CML）患者的一种有效方法。对于缺乏合适供体的患者，已经开发出多种利用从骨髓和/或外周血中获取的患者自身造血干细胞的方法。长期以来，人们观察到在长期骨髓培养（LTBMC）中，正常人造血祖细胞比CML克隆更易于恢复。从组织培养（TC）瓶到“生命细胞”袋（一种透气塑料袋，饲养层细胞无法附着）启动的LTBMC已经研究了数年。在我们实验室中，细胞在各种生长因子存在的情况下进行培养，以开发改进的干细胞扩增方法。已经观察到在透气塑料袋中无饲养层的情况下造血干细胞持续生长。对CML患者的骨髓生长情况正在进行广泛研究。在骨髓采集前联合使用有效药物以减少费城（Ph）克隆，并使用培养的骨髓，可能为治疗CML患者提供一种有用的方法。下面还将讨论各种国际研究的结果。
