Chung J G, Levy G N, Weber W W
Department of Pharmacology, University of Michigan, Ann Arbor 48109-0626.
Drug Metab Dispos. 1993 Nov-Dec;21(6):1057-63.
The distribution of N-acetyltransferase (NAT) activity in 35 tissues of inbred rapid acetylator C57BL/6J and slow acetylator congenic B6.A-NatS mice was determined by incubation of tissue cytosols with 2-aminofluorene or p-aminobenzoic acid followed by HPLC assay. Tissues examined included the gastrointestinal tract, lymphoid tissues, skin, blood components, and other major organs. NAT activity was found in all tissues examined except blood plasma and seminal vesicles. Peyer's patches had the highest activity with either substrate, and lymphoid tissue, in general, was high in NAT activity as was skin and much of the digestive system. The acetylator polymorphism was apparent in most tissues for both p-aminobenzoic acid and 2-aminofluorene. The difference between rapid and slow acetylator phenotypes was usually greater with p-aminobenzoic acid than with 2-aminofluorene. The presence of NAT in the 33 tissues of rapid and slow acetylator mice, as well as the absence of NAT in plasma and seminal vesicles, was confirmed by immunoblots using an anti-NAT antibody raised in rabbits. These results indicate the widespread distribution of NAT activity and the relative abundance of extrahepatic N-acetylation capacity in the mouse.
通过将组织胞质溶胶与2-氨基芴或对氨基苯甲酸一起孵育，然后进行高效液相色谱分析，测定了近交系快速乙酰化小鼠C57BL/6J和慢速乙酰化同源B6.A-NatS小鼠35种组织中N-乙酰基转移酶（NAT）的活性分布。所检测的组织包括胃肠道、淋巴组织、皮肤、血液成分和其他主要器官。除血浆和精囊外，在所检测的所有组织中均发现了NAT活性。派尔集合淋巴结对两种底物的活性最高，一般来说，淋巴组织、皮肤和大部分消化系统的NAT活性都很高。对氨基苯甲酸和2-氨基芴在大多数组织中都表现出乙酰化多态性。快速和慢速乙酰化表型之间的差异通常对氨基苯甲酸比2-氨基芴更大。使用兔抗NAT抗体进行免疫印迹证实了快速和慢速乙酰化小鼠的33种组织中存在NAT，以及血浆和精囊中不存在NAT。这些结果表明NAT活性广泛分布，且小鼠肝外N-乙酰化能力相对丰富。
