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[从俄罗斯不同地区分离出的伯纳特柯克斯体菌株的DNA限制性片段长度多态性特征]

[Characteristics of DNA restriction fragment length polymorphisms of C. burnetti strains, isolated from areas of Russia].

作者信息

Demkin V V, Tokarevich N K, Rydkina E B, Kuzina V A, Kartseva N A, Daĭter A B, Balaeva N M

出版信息

Mol Gen Mikrobiol Virusol. 1993 Nov-Dec(6):13-6.

PMID:7905598
Abstract

The structural heterogeneity in Coxiella burnetii chromosomal DNA isolated in the European part of Russia from people, agricultural animals, and ticks has been studied. It is compared with the one of the European strains Henzerling and M44, the only genetically characterized strains up to date. The digestion of the total DNA by the restriction endonucleases BamHI, PstI, XhoI resulted in obtaining two types of restriction patterns. The ones for Henzerling and M44 differed from the restriction patterns of the Russian strains, while the latter proved to be identical. The obtained data are in proof of genetical homogeneity in the Russian group of strains. The group is different from the genomic group including Henzerling and M44. The fact is in proof of the genetical heterogeneity of the European population of coxiellae.

摘要

对从俄罗斯欧洲部分的人、家畜和蜱中分离出的伯纳特柯克斯体染色体DNA的结构异质性进行了研究。将其与欧洲菌株亨泽林和M44(这是迄今为止仅有的经过基因特征鉴定的菌株)的结构异质性进行了比较。用限制性内切酶BamHI、PstI、XhoI消化总DNA,得到了两种类型的限制性图谱。亨泽林和M44的图谱与俄罗斯菌株的限制性图谱不同,而俄罗斯菌株的图谱则被证明是相同的。所得数据证明了俄罗斯菌株组的基因同质性。该菌株组与包括亨泽林和M44在内的基因组不同。这一事实证明了欧洲柯克斯体种群的基因异质性。

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