Pe'er J, Gnessin H, Shargal Y, Livni N
Department of Ophthalmology, Hadassah University Hospital, Jerusalem, Israel.
Ophthalmology. 1994 Jan;101(1):56-62. doi: 10.1016/s0161-6420(94)38024-9.
It is difficult to assess the viability of uveal melanoma after radiotherapy treatment. The purpose of the current study is to investigate PC-10 monoclonal antibody of proliferating cell nuclear antigen as a possible marker for cell proliferation and tumor viability in conventionally processed histologic preparations of uveal melanoma irradiated by brachytherapy as well as in nonirradiated melanomas.
Thirteen enucleated eyes with posterior uveal melanoma that were treated by brachytherapy (cobalt 60 or ruthenium 106 radioactive plaques) were included in this study. Thirteen enucleated eyes of the same size with nonirradiated posterior uveal melanoma served as controls. The tumors were stained with PC-10 monoclonal antibody to proliferating cell nuclear antigen. All clinical and histologic data of the tumors were recorded and analyzed.
Five of the irradiated tumors showed positive staining with PC-10, although with a low score. Four of these tumors showed regrowth, and the fifth tumor was treated with a low-irradiation dose (5500 rad). In the nonirradiated tumor group, nine were positive for PC-10 staining, with a higher score. Significant correlation was found in this group between the PC-10 score and the mitotic figure count, but not with other prognostic factors. In three of four tumors that caused metastatic death, the PC-10 staining was positive and had a high score.
PC-10 immunostaining is a simple, reproducible method that can be applicable to conventionally processed histologic preparations. It clearly shows that cellular proliferation activity in nonirradiated and irradiated uveal melanomas. Based on the small number of cases reported herein, it seems that the PC-10 score can correlate with prognosis, but further studies should be performed.
评估放疗后葡萄膜黑色素瘤的生存能力具有一定难度。本研究的目的是探讨增殖细胞核抗原的PC-10单克隆抗体，作为近距离放射治疗照射的葡萄膜黑色素瘤以及未照射黑色素瘤的常规处理组织学标本中细胞增殖和肿瘤生存能力的一种可能标志物。
本研究纳入了13只接受近距离放射治疗（钴60或钌106放射性敷贴）的后葡萄膜黑色素瘤摘除眼球。13只大小相同的未照射后葡萄膜黑色素瘤摘除眼球作为对照。肿瘤用增殖细胞核抗原的PC-10单克隆抗体进行染色。记录并分析肿瘤的所有临床和组织学数据。
5只接受照射的肿瘤显示PC-10阳性染色，尽管评分较低。其中4只肿瘤出现复发，第5只肿瘤接受了低剂量照射（5500拉德）。在未照射肿瘤组中，9只PC-10染色呈阳性，评分较高。该组中PC-10评分与有丝分裂计数之间存在显著相关性，但与其他预后因素无关。在导致转移死亡的4只肿瘤中的3只，PC-10染色呈阳性且评分较高。
PC-10免疫染色是一种简单、可重复的方法，可应用于常规处理的组织学标本。它清楚地显示了未照射和照射后葡萄膜黑色素瘤中的细胞增殖活性。基于本文报道的病例数量较少，似乎PC-10评分可能与预后相关，但应进行进一步研究。
