用于排除Rb基因突变携带者状态的限制性片段长度多态性聚合酶链反应技术
[RFLP-PCR technique for exclusion of carrier status of the mutated Rb gene].
作者信息
Zajaczek S, Podolski J, Lubiński J, Rosławska A, Krzystolik Z, Sagan Z
机构信息
Zakładu Genetyki i Patomorfologii Instytutu Patologii, Pomorskiej AM.
出版信息
Klin Oczna. 1993 Jun;95(6):216-8.
PMID:7905940
Abstract
Molecular-genetic analysis of DNA by PCR-RFLP technique in a family with a case of unilateral retinoblastoma was performed. The carrier status of mutated Rb gene in a sister of proband was excluded on the basis of molecular analysis despite unsynonymous pedigree data.
摘要
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对一个患有单侧视网膜母细胞瘤的家族进行了DNA分子遗传学分析。尽管系谱数据存在不确定性，但根据分子分析排除了先证者姐妹中突变Rb基因的携带者状态。
Zotero 插件