Identification of a chaperonin-10 homologue in plant mitochondria.

Chaperonin-60 and chaperonin-10 form stable binary complexes in the presence of ATP. This phenomenon has been used as the basis for the identification of a chaperonin-10 homologue in potato (Solanum tuberosum L.) mitochondria. ATP-dependent binary complexes formed between chaperonin-60 and chaperonin-10 in potato mitochondrial extracts were isolated by affinity chromatography on a column of immobilised chaperonin-60 antibodies. Partial amino acid sequence analysis of the chaperonin-10 protein indicates that it is related in primary structure to rat mitochondrial chaperonin-10.