Hayamizu T F, Wanek N, Taylor G, Trevino C, Shi C, Anderson R, Gardiner D M, Muneoka K, Bryant S V
Developmental Biology Center, University of California, Irvine 92717.
Dev Biol. 1994 Feb;161(2):504-12. doi: 10.1006/dbio.1994.1048.
The expression domains of genes located at the 5' end of the HoxD (formerly Hox-4) complex appear to correlate with pattern along both the proximal-distal (PrDi) and the anterior-posterior (AP) axes of the developing limb bud, and it has been suggested that the HoxD gene products are involved in the specification of positional information during limb development. The apical ectodermal ridge is required for limb outgrowth and is thought to influence mesodermal cells at the distal end of the limb bud in a region within which patterning events occur. In this paper, we examine the expression of 5' HoxD genes during PrDi pattern regulation in chick wing buds. In limbs undergoing pattern regulation, we demonstrate that the domains of HoxD11 and HoxD13 gene expression are "regenerated" within 24 hr of removal of the distal mesenchyme. In contrast, in limbs which will not form distal structures, HoxD13 expression becomes reduced.
位于HoxD（原Hox - 4）复合体5'端的基因表达域，似乎与发育中的肢芽沿近远轴（PrDi）和前后轴（AP）的模式相关，并且有人提出HoxD基因产物参与肢体发育过程中位置信息的特化。顶外胚层嵴是肢体生长所必需的，并且被认为在肢体芽远端的中胚层细胞中，在发生模式形成事件的区域内影响这些细胞。在本文中，我们研究了鸡翼芽PrDi模式调节过程中5'HoxD基因的表达。在经历模式调节的肢体中，我们证明在去除远端间充质后的24小时内，HoxD11和HoxD13基因表达域会“再生”。相比之下，在不会形成远端结构的肢体中，HoxD13表达会降低。
