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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Kokubu N, Satoh M, Takayanagi I

Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences, Chiba, Japan.

Gen Pharmacol. 1993 Nov;24(6):1541-5. doi: 10.1016/0306-3623(93)90449-8.

The mechanisms involved in contraction of rabbit iris dilator muscle induced by norepinephrine (NE) were studied. 2. The concentration-response curve of NE was not influenced by Ca2+ blockers in the normal physiological saline solution (PSS) and removal of Ca2+ from PSS. 3. In 0.01 mM EGTA containing Ca(2+)-free PSS, the NE-induced contraction was phasic, which was suppressed by TMB-8, cyclopiazonic acid, ionomycin and A23187 but still partly remained. 4. In 2 mM EGTA containing Ca(2+)-free PSS, NE increased the intracellular Ca2+ ([Ca2+]i) and muscle tension. Ryanodine abolished the increase in [Ca2+]i induced by NE but slightly inhibited the tension. 5. These results suggest that the NE-induced contraction of rabbit iris dilator in normal PSS is mainly due to the increase in the release of intracellularly sequestered Ca2+ and partly due to the Ca(2+)-independent processes.

研究了去甲肾上腺素（NE）诱导家兔虹膜开大肌收缩的机制。2. 在正常生理盐溶液（PSS）中以及从PSS中去除Ca2+时，NE的浓度-反应曲线不受Ca2+阻滞剂的影响。3. 在含有0.01 mM乙二醇双四乙酸（EGTA）的无Ca2+ PSS中，NE诱导的收缩是阶段性的，可被三甲基溴化铵（TMB-8）、环匹阿尼酸、离子霉素和A23187抑制，但仍部分存在。4. 在含有2 mM EGTA的无Ca2+ PSS中，NE增加了细胞内Ca2+（[Ca2+]i）和肌肉张力。ryanodine消除了NE诱导的[Ca2+]i增加，但轻微抑制了张力。5. 这些结果表明，在正常PSS中，NE诱导的家兔虹膜开大肌收缩主要是由于细胞内储存的Ca2+释放增加，部分是由于与Ca2+无关的过程。

Kokubu N, Satoh M, Takayanagi I

Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences, Chiba, Japan.

Gen Pharmacol. 1993 Nov;24(6):1541-5. doi: 10.1016/0306-3623(93)90449-8.

The mechanisms involved in contraction of rabbit iris dilator muscle induced by norepinephrine (NE) were studied. 2. The concentration-response curve of NE was not influenced by Ca2+ blockers in the normal physiological saline solution (PSS) and removal of Ca2+ from PSS. 3. In 0.01 mM EGTA containing Ca(2+)-free PSS, the NE-induced contraction was phasic, which was suppressed by TMB-8, cyclopiazonic acid, ionomycin and A23187 but still partly remained. 4. In 2 mM EGTA containing Ca(2+)-free PSS, NE increased the intracellular Ca2+ ([Ca2+]i) and muscle tension. Ryanodine abolished the increase in [Ca2+]i induced by NE but slightly inhibited the tension. 5. These results suggest that the NE-induced contraction of rabbit iris dilator in normal PSS is mainly due to the increase in the release of intracellularly sequestered Ca2+ and partly due to the Ca(2+)-independent processes.

研究了去甲肾上腺素（NE）诱导家兔虹膜开大肌收缩的机制。2. 在正常生理盐溶液（PSS）中以及从PSS中去除Ca2+时，NE的浓度-反应曲线不受Ca2+阻滞剂的影响。3. 在含有0.01 mM乙二醇双四乙酸（EGTA）的无Ca2+ PSS中，NE诱导的收缩是阶段性的，可被三甲基溴化铵（TMB-8）、环匹阿尼酸、离子霉素和A23187抑制，但仍部分存在。4. 在含有2 mM EGTA的无Ca2+ PSS中，NE增加了细胞内Ca2+（[Ca2+]i）和肌肉张力。ryanodine消除了NE诱导的[Ca2+]i增加，但轻微抑制了张力。5. 这些结果表明，在正常PSS中，NE诱导的家兔虹膜开大肌收缩主要是由于细胞内储存的Ca2+释放增加，部分是由于与Ca2+无关的过程。