Zhang X, Colombel M, Raffo A, Buttyan R
Department of Urology, Columbia University, New York, NY 10032.
Biochem Biophys Res Commun. 1994 Feb 15;198(3):1189-94. doi: 10.1006/bbrc.1994.1168.
Previously, increased expression of mRNA encoding the p53 tumor suppressor protein was described during castration-induced regression of the rat ventral prostate gland with Northern blot techniques. This activity was confirmed with a ribonuclease protection assay that demonstrated a 16-fold induction of p53 transcripts in ventral prostate RNA within 72 hrs after castration. The induced expression of p53 mRNA correlated with increased detection of p53 protein in nuclei of regressing prostate epithelial cells. Immunohistochemical staining with anti-p53 antibody was strongly reactive for epithelial nuclei in castrated glands but unreactive for nuclei of control adult glands. In contrast to the upregulation of p53 in regressing prostate glands with a large proportion of apoptotic cells, expression of p53 mRNA was decreased in rat prostate glands that were stimulated to regrow by testosterone replacement.
此前，利用Northern印迹技术在大鼠腹侧前列腺去势诱导的退化过程中，发现编码p53肿瘤抑制蛋白的mRNA表达增加。核糖核酸酶保护试验证实了这一活性，该试验表明去势后72小时内腹侧前列腺RNA中p53转录本诱导增加了16倍。p53 mRNA的诱导表达与退化前列腺上皮细胞核中p53蛋白检测增加相关。用抗p53抗体进行免疫组织化学染色显示，去势腺体的上皮细胞核呈强阳性反应，而对照成年腺体的细胞核无反应。与具有大量凋亡细胞的退化前列腺中p53上调相反，用睾酮替代刺激再生的大鼠前列腺中p53 mRNA表达降低。
