急性髓系白血病的白血病原始细胞与细胞毒性淋巴细胞形成共轭体。

Conjugate formation by leukemic blasts from acute myeloid leukemia with cytotoxic lymphocytes.

作者信息

Palucka A K, Porwit A, Reizenstein P

机构信息

Department of Pathology, Karolinska Hospital, Stockholm, Sweden.

出版信息

Leuk Lymphoma. 1993 Nov;11(5-6):427-33. doi: 10.3109/10428199309067936.

DOI:10.3109/10428199309067936

PMID:7907248

Abstract

Conjugate formation by AML blasts with fresh peripheral blood lymphocytes (PBL) and lymphokine activated killer (LAK) effectors was studied by flow cytometry. Leukemic blasts formed very low numbers of conjugates with fresh PBL and were resistant to natural killer (NK) cytotoxicity. When LAK effectors were used a significant increase in conjugate formation was observed, which in the majority of cases was followed by an increased killing. There was a positive correlation between the percentages of conjugates formed by AML blasts with LAK effectors and the susceptibility to lysis. No significant difference in binding activity between the CD3+ and CD56+ LAK subpopulations was found. There was no correlation between the expression of ICAM-1, LFA-3 and Transferrin receptor (CD71) and the conjugate formation. The blocking of CD71 on the control K562 cell line reduced the conjugate formation with LAK effectors but no such effect could be observed with CD71+ AML blasts.

摘要

通过流式细胞术研究急性髓系白血病（AML）原始细胞与新鲜外周血淋巴细胞（PBL）以及淋巴因子激活的杀伤细胞（LAK）效应细胞形成共轭物的情况。白血病原始细胞与新鲜PBL形成的共轭物数量非常少，并且对自然杀伤（NK）细胞毒性具有抗性。当使用LAK效应细胞时，观察到共轭物形成显著增加，在大多数情况下随后杀伤作用增强。AML原始细胞与LAK效应细胞形成的共轭物百分比与裂解敏感性之间存在正相关。在CD3 +和CD56 + LAK亚群之间未发现结合活性有显著差异。细胞间黏附分子-1（ICAM-1）、淋巴细胞功能相关抗原-3（LFA-3）和转铁蛋白受体（CD71）的表达与共轭物形成之间无相关性。在对照K562细胞系上阻断CD71可减少与LAK效应细胞的共轭物形成，但在CD71 + AML原始细胞上未观察到这种效应。