Vickers S E, Warr J R
Biology Department, University of York, UK.
Cancer Lett. 1994 Jan 15;76(1):51-6. doi: 10.1016/0304-3835(94)90133-3.
Cell viability and P-glycoprotein expression in Chinese hamster ovary cells have been measured following incubation in a range of vincristine concentrations for 24 h. An increase of 12% in cell viability was observed with increasing drug concentrations in a drug-sensitive cell line (E29) and a lesser increase of 5% in a multidrug-resistant cell line (VRA15). The increased cell viability corresponded to a rise of P-glycoprotein level. Slot blots showed that there was an increase in P-glycoprotein mRNA with all drug concentrations tested in E29, although there was only partial correlation of mRNA levels with P-glycoprotein levels viability.
将中国仓鼠卵巢细胞在一系列长春新碱浓度下孵育24小时后，测定了细胞活力和P-糖蛋白表达。在药物敏感细胞系（E29）中，随着药物浓度增加，观察到细胞活力增加了12%，而在多药耐药细胞系（VRA15）中增加较少，为5%。细胞活力的增加与P-糖蛋白水平的升高相对应。狭缝印迹显示，在E29中，所有测试药物浓度下P-糖蛋白mRNA均增加，尽管mRNA水平与P-糖蛋白水平活力之间仅存在部分相关性。
