Brown W C, Zhao S, Woods V M, Dobbelaere D A, Rice-Ficht A C
Department of Veterinary Pathobiology, Texas A & M University, College Station.
Rev Elev Med Vet Pays Trop. 1993;46(1-2):65-9.
The central role of T cells in the immune response against hemoprotozoan parasites, both as helper cells for T-dependent antibody production, and as effector cells acting directly or indirectly on intracellular parasites through the elaboration of cytokines, has prompted us to investigate the bovine cellular immune response against B. bovis antigens. T cell clones generated from four B. bovis-immune cattle by in vitro stimulation with soluble or membrane associated merozoite antigen were characterized for reactivity against various forms of antigen and different geographical isolates of B. bovis and B. bigemina. The clones were categorized into seven different groups based on differential patterns of reactivity. This panel of T cell clones and additional clones specific for either the 77 kDa merozoite apical complex associated protein (Bb-1) or the 42 kDa major merozoite protein (MSA-1) were analyzed for cytokines. Biological assays to measure IL-2/IL-4, IFN-gamma and TNF-alpha/TNF-beta and Northern blot analysis to detect mRNA encoding bovine IL2, IL-4, IFN-gamma, TNF-beta and TNF-alpha revealed the differential production of cytokines by clones with different antigen specificities. Two Bb-1-specific T cell clones produced the Th1 pattern of cytokines: IL-2, IFN-gamma, TNF-beta and TNF-alpha, but not IL-4. Clones specific for the 42 kDa protein produced undetectable levels of all cytokines, but expressed an unrestricted or Th0 pattern of cytokine mRNA: IL-2, IL-4, IFN-gamma and TNF-alpha.(ABSTRACT TRUNCATED AT 250 WORDS)
T细胞在针对血液原虫寄生虫的免疫反应中发挥着核心作用，它既是产生T细胞依赖性抗体的辅助细胞，又是通过分泌细胞因子直接或间接作用于细胞内寄生虫的效应细胞。这促使我们研究牛对牛巴贝斯虫抗原的细胞免疫反应。通过用可溶性或膜相关裂殖子抗原进行体外刺激，从四头感染牛巴贝斯虫的牛产生的T细胞克隆，针对各种形式的抗原以及牛巴贝斯虫和双芽巴贝斯虫的不同地理分离株的反应性进行了表征。根据不同的反应模式，这些克隆被分为七个不同的组。对这组T细胞克隆以及针对77 kDa裂殖子顶端复合物相关蛋白（Bb-1）或42 kDa主要裂殖子蛋白（MSA-1）的其他克隆进行了细胞因子分析。用于测量IL-2/IL-4、IFN-γ和TNF-α/TNF-β的生物学测定以及用于检测编码牛IL2、IL-4、IFN-γ、TNF-β和TNF-α的mRNA的Northern印迹分析显示，具有不同抗原特异性的克隆产生细胞因子的情况存在差异。两个Bb-1特异性T细胞克隆产生Th1型细胞因子：IL-2、IFN-γ、TNF-β和TNF-α，但不产生IL-4。针对42 kDa蛋白的克隆产生的所有细胞因子水平均无法检测到，但表达不受限制的或Th0型细胞因子mRNA：IL-2、IL-4、IFN-γ和TNF-α。（摘要截短于250字）
