Pratviel-Sosa F, Acher F, Trigalo F, Blanot D, Azerad R, van Heijenoort J
Enveloppes Bactériennes et Peptides, URA 1131 du CNRS, Université de Paris-Sud, Orsay, France.
FEMS Microbiol Lett. 1994 Jan 15;115(2-3):223-8. doi: 10.1111/j.1574-6968.1994.tb06642.x.
Twenty-four analogues of D-glutamic acid were tested as substrates or inhibitors of the D-glutamate-adding enzyme from Escherichia coli. The best substrates were, in decreasing order of specific activity, D-erythro-4-methylglutamic acid, D-erythro-3-methylglutamic acid, DL-homocysteic acid, (+/-)-trans-1-amino-3-carboxy-cyclopentanecarboxylic acid and (+/-)-trans-1-amino-3-carboxy-cyclohexanecarboxylic acid. Among the different stereoisomers, only the D-erythro isomers for methylglutamic acids, and the trans isomers for the cyclic analogs, were substrates. Apart from the D-erythro-3- and 4-methylglutamic acids and DL-homocysteic acid, none of the examined compounds significantly inhibited the addition of radioactive D-glutamic acid to UDP-N-acetylmuramyl-L-alanine.
对24种D-谷氨酸类似物作为大肠杆菌D-谷氨酸添加酶的底物或抑制剂进行了测试。按比活性从高到低的顺序，最佳底物依次为D-赤藓糖-4-甲基谷氨酸、D-赤藓糖-3-甲基谷氨酸、DL-高胱氨酸、(±)-反式-1-氨基-3-羧基环戊烷羧酸和(±)-反式-1-氨基-3-羧基环己烷羧酸。在不同的立体异构体中，只有甲基谷氨酸的D-赤藓糖异构体和环状类似物的反式异构体是底物。除了D-赤藓糖-3-和4-甲基谷氨酸以及DL-高胱氨酸外，所检测的化合物均未显著抑制放射性D-谷氨酸添加到UDP-N-乙酰胞壁酰-L-丙氨酸中。
