[运用细胞遗传学和分子技术检测（15；17）易位。15例急性早幼粒细胞白血病患者的比较研究]
[Detection of translocation (15;17) using cytogenetic and molecular techniques. Comparative study in 15 patients with acute promyelocytic leukemia].
作者信息
Arranz E, Benítez J, Santón A, Portero J A, Sánchez-Fayos J
机构信息
Departamento de Genética y Hematología, Fundación Jiménez Díaz, Madrid.
出版信息
Sangre (Barc). 1993 Oct;38(5):355-8.
PURPOSES
To analyze the reproducibility to detect the t (15; 17) with molecular and cytogenetic techniques.
PATIENTS AND METHODS
Fifteen cases of acute promyelocytic leukaemia were studied by means of cytogenetic techniques (GTG banding) and molecular ones (K/S probe and HindIII, EcoRI and BamHI restriction enzymes).
RESULTS
The t (15; 17) was detected with cytogenetic techniques in 60% cases and with molecular ones in 33% cases. Using both (cytogenetic and molecular) techniques the translocation was detected in 73% cases.
CONCLUSIONS
The use of both techniques may be very useful in order to get a better diagnostic and a closer control of the patient's clinical evolution.
目的
分析运用分子和细胞遗传学技术检测t（15；17）的可重复性。
患者与方法
采用细胞遗传学技术（GTG显带）和分子技术（K/S探针以及HindIII、EcoRI和BamHI限制性内切酶）对15例急性早幼粒细胞白血病患者进行研究。
结果
细胞遗传学技术检测到t（15；17）的病例占60%，分子技术检测到的占33%。同时使用（细胞遗传学和分子）两种技术时，检测到易位的病例占73%。
结论
为了更好地进行诊断并密切监测患者的临床病情进展，同时使用这两种技术可能非常有用。
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