Koyama S
Department of Internal Medicine, University of Tsukuba, Ibaraki.
Jpn J Cancer Res. 1994 Feb;85(2):131-4. doi: 10.1111/j.1349-7006.1994.tb02072.x.
We have examined whether shedding intercellular adhesion molecule-1 (ICAM-1) antigen from cultured tumors is able to inhibit the leukocyte function-associated antigen-1 (LFA-1)/ICAM-1 interaction between cytotoxic effector cells and ICAM-1+ target tumor cells. The cytotoxic activity of lymphokine-activated killer (LAK) cells incubated with spent media from ICAM-1+ tumor cells, especially interferon-gamma-stimulated tumor cells, was significantly decreased as compared with that of LAK cells treated with fresh culture medium without ICAM-1 antigen. Treatment of LAK cells with spent media from ICAM-1- tumor cells did not cause a significant decrease of the cytolytic activity towards ICAM-1+ tumor cells. These findings suggest that shedding of ICAM-1 antigen could be involved in binding of LFA-1 to LAK cells, resulting in reduced cytolytic activity.
我们研究了培养的肿瘤细胞脱落的细胞间黏附分子-1（ICAM-1）抗原是否能够抑制细胞毒性效应细胞与ICAM-1⁺靶肿瘤细胞之间的白细胞功能相关抗原-1（LFA-1）/ICAM-1相互作用。与用不含ICAM-1抗原的新鲜培养基处理的淋巴因子激活的杀伤（LAK）细胞相比，与ICAM-1⁺肿瘤细胞（尤其是干扰素-γ刺激的肿瘤细胞）的用过的培养基一起孵育的LAK细胞的细胞毒性活性显著降低。用ICAM-1⁻肿瘤细胞的用过的培养基处理LAK细胞不会导致对ICAM-1⁺肿瘤细胞的细胞溶解活性显著降低。这些发现表明，ICAM-1抗原的脱落可能参与LFA-1与LAK细胞的结合，导致细胞溶解活性降低。
