Goldstein R E, Wasserman D H, Reed G W, Lacy D B, Abumrad N N, Cherrington A D
Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.
Horm Metab Res. 1994 Jan;26(1):9-13. doi: 10.1055/s-2007-1000764.
The present study was undertaken to determine whether an acute physiologic rise in plasma cortisol during selective insulin deficiency would have significant effects on glycerol and beta-hydroxybutyrate metabolism in conscious overnight-fasted dogs. Each experiment consisted of a two hour dye equilibration period, a 40 minute basal period, and a 3 hour experimental period. A continuous infusion of indocyanine green dye for blood flow estimation was initiated at the start of the equilibration period and continued throughout the experiment. In both of two protocols selective insulin deficiency was created during the experimental period by infusing somatostatin peripherally (0.8 microgram/kg-min) with basal replacement of glucagon intraportally (0.65 ng/kg-min). In the test protocol (CORTISOL, n = 5), 3.0 micrograms/kg-min of hydrocortisone was infused during the experimental period. In the control protocol (SALINE, n = 5), saline was infused. Net hepatic balances were determined using the (A-V) difference technique. During selective insulin deficiency alone (SALINE), the arterial blood glycerol level increased from 81 +/- 19 to 140 +/- 11 microM (p < 0.01) and net hepatic glycerol uptake (NHGlyU) tended to increase from 2.3 +/- 0.3 to 3.3 +/- 0.6 mumol/kg-min (0.05 < 0.1). The arterial plasma free fatty acid (FFA) level remained unchanged at 1041 +/- 35 microM. The arterial beta-hydroxybutyrate (BHOB) level increased slightly from 21 +/- 4 to 29 +/- 5 microM while net hepatic beta-hydroxybutyrate production (NHBP) remained unchanged (1.0 +/- 0.2 mumol/kg-min). During acute hypercortisolemia with selective insulin deficiency (CORTISOL), similar changes occurred in the arterial blood glycerol level and net hepatic glycerol uptake.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在确定在选择性胰岛素缺乏期间血浆皮质醇的急性生理性升高是否会对清醒禁食过夜犬的甘油和β-羟基丁酸代谢产生显著影响。每个实验包括两个小时的染料平衡期、40分钟的基础期和3小时的实验期。在平衡期开始时启动持续输注吲哚菁绿染料以估计血流量，并在整个实验过程中持续进行。在两个方案中，在实验期通过外周输注生长抑素（0.8微克/千克-分钟）并经门静脉基础替代胰高血糖素（0.65纳克/千克-分钟）来造成选择性胰岛素缺乏。在测试方案（皮质醇组，n = 5）中，在实验期输注3.0微克/千克-分钟的氢化可的松。在对照方案（生理盐水组，n = 5）中，输注生理盐水。使用（A-V）差值技术测定肝脏净平衡。仅在选择性胰岛素缺乏期间（生理盐水组），动脉血甘油水平从81±19微摩尔/升升至140±11微摩尔/升（p < 0.01），肝脏甘油净摄取量（NHGlyU）倾向于从2.3±0.3微摩尔/千克-分钟增至3.3±0.6微摩尔/千克-分钟（0.05 < p < 0.1）。动脉血浆游离脂肪酸（FFA）水平保持不变，为1041±35微摩尔/升。动脉β-羟基丁酸（BHOB）水平从21±4微摩尔/升略有升至29±5微摩尔/升，而肝脏β-羟基丁酸净生成量（NHBP）保持不变（1.0±0.2微摩尔/千克-分钟）。在伴有选择性胰岛素缺乏的急性高皮质醇血症期间（皮质醇组），动脉血甘油水平和肝脏甘油净摄取量发生了类似变化。（摘要截断于250字）
