Warburton A L, Santer R M
Department of Anatomy, University of Wales College of Cardiff, UK.
Histochem J. 1994 Feb;26(2):127-33. doi: 10.1007/BF00157961.
The sympathetic innervation of the urinary tract of young adult (4 months) and aged (24+ months) rats has been examined by glyoxylic acid-induced fluorescence for the detection of noradrenaline and by immunofluorescence using antisera against tyrosine hydroxylase (TH) and neuropeptide Y (NPY). Immunostaining for calcitonin gene-related peptide (CGRP), known to be present in pelvic sensory nerves, was also performed. Semi-quantitative estimations of nerve densities were made of noradrenergic and peptidergic fibres innervating the smooth musculature of the ureter, bladder and urethra, and of the urinary tract vasculature. In the aged rats the overall patterns of innervation remained unchanged. However, with the exception of the vesical vasculature, the density of noradrenergic innervation decreased as did the intensity of histofluorescence. A similar pattern of results was observed by TH and NPY immunofluorescence. The results present evidence for a diminution in the sympathetic control of the urinary tract in aged rats. The pattern and density of CGRP-immunoreactive nerves was unchanged in the aged animals suggesting that pelvic visceral sensory innervation is more resistant to the effects of advancing age.
通过用乙醛酸诱导荧光检测去甲肾上腺素以及使用抗酪氨酸羟化酶（TH）和神经肽Y（NPY）的抗血清进行免疫荧光，研究了年轻成年（4个月）和老年（24个月以上）大鼠尿路的交感神经支配。还对已知存在于盆腔感觉神经中的降钙素基因相关肽（CGRP）进行了免疫染色。对支配输尿管、膀胱和尿道平滑肌以及尿路血管系统的去甲肾上腺素能和肽能纤维的神经密度进行了半定量估计。在老年大鼠中，总体神经支配模式保持不变。然而，除了膀胱血管系统外，去甲肾上腺素能神经支配的密度降低，组织荧光强度也降低。TH和NPY免疫荧光观察到类似的结果模式。结果表明老年大鼠尿路的交感神经控制减弱。老年动物中CGRP免疫反应性神经的模式和密度没有变化，这表明盆腔内脏感觉神经支配对衰老的影响更具抵抗力。
