Felten S Y, Bellinger D L, Collier T J, Coleman P D, Felten D L
Neurobiol Aging. 1987 Mar-Apr;8(2):159-65. doi: 10.1016/0197-4580(87)90026-1.
Splenic noradrenergic innervation in young adult and aged Fischer 344 rats was examined using fluorescence histochemistry for catecholamines and high performance liquid chromatography with electrochemical detection (LCEC) for the quantitation of norepinephrine (NE). In young adult rats, abundant noradrenergic plexuses followed the vasculature and trabeculae into splenic white pulp. In aged rats, noradrenergic innervation was reduced in density and in overall intensity of fluorescence, and splenic NE levels were significantly lower. The relationship between diminished noradrenergic innervation and diminished immune responsiveness in aging mammals, while not clear on a causal level, is presented as a hypothesis for further testing.
利用儿茶酚胺荧光组织化学法以及采用电化学检测的高效液相色谱法（LCEC）对去甲肾上腺素（NE）进行定量分析，研究了成年和老年Fischer 344大鼠脾脏的去甲肾上腺素能神经支配情况。在成年大鼠中，丰富的去甲肾上腺素能神经丛沿着血管和小梁进入脾脏白髓。在老年大鼠中，去甲肾上腺素能神经支配的密度和荧光总体强度降低，脾脏NE水平显著降低。去甲肾上腺素能神经支配减少与衰老哺乳动物免疫反应性降低之间的关系，虽然在因果层面尚不清楚，但作为一个假设提出以供进一步检验。
