脑源性神经营养因子预处理对培养的大鼠皮质神经元中谷氨酸诱导的神经毒性具有部分保护作用。

Brain-derived neurotrophic factor pretreatment exerts a partially protective effect against glutamate-induced neurotoxicity in cultured rat cortical neurons.

作者信息

Shimohama S, Tamura Y, Akaike A, Tsukahara T, Ohara O, Watanabe S, Kimura J

机构信息

Department of Neurology, Faculty of Medicine, Kyoto University, Japan.

出版信息

Neurosci Lett. 1993 Dec 24;164(1-2):55-8. doi: 10.1016/0304-3940(93)90856-g.

DOI:10.1016/0304-3940(93)90856-g

PMID:7908729

Abstract

The effect of recombinant brain-derived neurotrophic factor (BDNF) on cultured rat cortical neurons was examined. Brief exposure of cortical neurons to glutamate followed by incubation with glutamate-free medium reduced cell viability by 60-70% when compared with the control value. Simultaneous addition of recombinant BDNF to rat cortical cultures with glutamate did not affect this reduction of cell viability. However, 24 h pretreatment of rat cortical cultures with recombinant BDNF resulted in a significant reduction of glutamate-induced neuronal damage. These findings suggest that BDNF can protect cortical neurons against glutamate-induced neurotoxicity.

摘要

研究了重组脑源性神经营养因子（BDNF）对培养的大鼠皮层神经元的影响。与对照组相比，皮层神经元短暂暴露于谷氨酸后，再在无谷氨酸培养基中孵育，细胞活力降低了60 - 70%。在含谷氨酸的大鼠皮层培养物中同时添加重组BDNF，并未影响细胞活力的这种降低。然而，用重组BDNF对大鼠皮层培养物进行24小时预处理，可显著降低谷氨酸诱导的神经元损伤。这些发现表明，BDNF可以保护皮层神经元免受谷氨酸诱导的神经毒性。