Premawansa S, Gamage-Mendis A, Perera L, Begarnie S, Mendis K, Carter R
Institute of Cell, Animal and Population Biology, University of Edinburgh, Scotland, UK.
Parasite Immunol. 1994 Jan;16(1):35-42. doi: 10.1111/j.1365-3024.1994.tb00302.x.
Sera from acute primary Plasmodium falciparum patients in Sri Lanka were tested for the presence of antibodies against gamete antigens and for their functional effects of transmission blocking activity. Comparisons were made with corresponding data from a previous study from sera of patients from Papua New Guinea where malaria is more highly endemic. Although the prevalence of anti-gamete antibodies in the two groups were broadly similar, the prevalence of infectivity suppressive effects in the Sri Lankan sera (56%) was less than in Papua New Guinea sera (75%), suggesting that the generation of functionally effective transmission blocking antibodies requires prolonged exposure to multiple inoculations of malaria. In Papua New Guinea sera there was a good correlation between transmission blocking effects and antibody responses to Pfs 230, a known target of transmission blocking antibodies. Among the Sri Lankan sera no strong correlation was found between transmission blocking effects and the presence of antibodies to gamete surface antigens Pfs 230 nor Pfs 48/45 as detected by immunoprecipitation of radio-iodinated gamete proteins; a strong correlation was however, found between the intensity of response to gamete surface antigens by IFA and transmission blocking effects of these sera. It is possible therefore, that the antigens identified by IFA include non-protein moieties and that these may be the targets of transmission blocking antibodies in sera from acute primary infections of P. falciparum.
对来自斯里兰卡急性初发恶性疟原虫患者的血清进行检测，以确定是否存在针对配子抗原的抗体及其传播阻断活性的功能效应。并与先前一项针对疟疾流行程度更高的巴布亚新几内亚患者血清的研究中的相应数据进行比较。尽管两组中抗配子抗体的流行率大致相似，但斯里兰卡血清中感染性抑制效应的流行率（56%）低于巴布亚新几内亚血清（75%），这表明产生功能有效的传播阻断抗体需要长期多次接触疟疾感染。在巴布亚新几内亚血清中，传播阻断效应与对Pfs 230（一种已知的传播阻断抗体靶点）的抗体反应之间存在良好的相关性。在斯里兰卡血清中，通过放射性碘化配子蛋白的免疫沉淀检测，未发现传播阻断效应与针对配子表面抗原Pfs 230或Pfs 48/45的抗体存在之间有强相关性；然而，通过间接荧光抗体试验（IFA）检测发现，对配子表面抗原反应的强度与这些血清的传播阻断效应之间存在强相关性。因此，有可能IFA鉴定出的抗原包括非蛋白质部分，并且这些可能是恶性疟原虫急性初发感染血清中传播阻断抗体的靶点。
