Sung C P, Arleth A J, Nambi P
Department of Cardiovascular Pharmacology and Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pa 19406.
Pharmacology. 1994 Mar;48(3):143-6. doi: 10.1159/000139173.
Potent activators of protein kinase C (PKC), such as phorbol dibutyrate and octylindolactam V, stimulated expression of intercellular adhesion molecule 1 (ICAM-1) by human umbilical vein endothelial cells (HUVEC) in a concentration-dependent manner. Expression of PKC activator-induced ICAM-1 in HUVEC was inhibited by the PKC inhibitor, H-7. Furthermore, cytokine (TNF alpha, LPS)-induced ICAM-1 expression was inhibited by the potent PKC inhibitor, H-7, and not by the cAMP-dependent protein kinase (PKA) specific inhibitor, H-89. These data suggest that PKC is involved in cytokine- and inflammatory agent-induced upregulation of ICAM-1 expression in HUVEC.
蛋白激酶C（PKC）的强效激活剂，如佛波醇二丁酸酯和辛基吲哚酰胺V，可浓度依赖性地刺激人脐静脉内皮细胞（HUVEC）表达细胞间黏附分子1（ICAM-1）。PKC抑制剂H-7可抑制PKC激活剂诱导的HUVEC中ICAM-1的表达。此外，强效PKC抑制剂H-7可抑制细胞因子（TNFα、LPS）诱导的ICAM-1表达，而cAMP依赖性蛋白激酶（PKA）特异性抑制剂H-89则无此作用。这些数据表明，PKC参与了细胞因子和炎症因子诱导的HUVEC中ICAM-1表达上调。
