沙眼衣原体小鼠肺炎病原体groES和groEL基因的序列。

The sequence of the groES and groEL genes from the mouse pneumonitis agent of Chlamydia trachomatis.

作者信息

Ho Y, Zhang Y X

机构信息

Maxwell Finland Laboratory for Infectious Diseases, Boston City Hospital, Boston University School of Medicine, MA 02118.

出版信息

Gene. 1994 Apr 8;141(1):143-4. doi: 10.1016/0378-1119(94)90145-7.

DOI:10.1016/0378-1119(94)90145-7

PMID:7909303

Abstract

The co-transcribed structural genes, groES and groEL, of the groE stress response operon from Chlamydia trachomatis mouse pneumonitis (MoPn), were cloned and sequenced. The calculated molecular masses of the encoded heat-shock proteins (Hsp), a small Hsp (GroES) and Hsp60 (GroEL), are 11,089 and 58,367 Da, respectively. By comparison with other known chlamydial groES and groEL sequences, there is 89 and 94% nucleotide (nt) identity with C. trachomatis human strains (serovars A and L2), 77 and 82% with C. psittaci strain GPIC, and 75 and 80% with C. pneumoniae isolate AR-39. At the amino-acid level, the MoPn Hsp60 shows a 99% identity with those from C. trachomatis human strains. In a mouse model, MoPn Hsp60 could prove useful in deciphering the pathogenesis of human chlamydial diseases.

摘要

对沙眼衣原体小鼠肺炎（MoPn）的groE应激反应操纵子的共转录结构基因groES和groEL进行了克隆和测序。所编码的热休克蛋白（Hsp），即小分子Hsp（GroES）和Hsp60（GroEL）的计算分子量分别为11,089和58,367道尔顿。与其他已知的衣原体groES和groEL序列相比，与沙眼衣原体人源菌株（血清型A和L2）的核苷酸（nt）同一性为89%和94%，与鹦鹉热衣原体菌株GPIC的同一性为77%和82%，与肺炎衣原体分离株AR-39的同一性为75%和80%。在氨基酸水平上，MoPn Hsp60与沙眼衣原体人源菌株的Hsp60有99%的同一性。在小鼠模型中，MoPn Hsp60可能有助于阐明人类衣原体疾病的发病机制。