Nakajima Y, Konno S, Perruccio L, Chen Y, Wu J M, An S, Chiao J, Choudhury M, Mallouh C, Muraki J
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Biochem Biophys Res Commun. 1994 Apr 15;200(1):467-74. doi: 10.1006/bbrc.1994.1472.
Addition of IFN-beta resulted in a dose-dependent reduction of growth, a drop in [3H]thymidine incorporation into DNA, and a concurrent 69% and 15% increase in the S and G2/M phases, of the human prostatic JCA-1 cells. No correlation existed between the antimitogenicity of IFN and increases in the double-stranded RNA-dependent protein kinase activity. Although IFN elicited a large increase in 2-5A synthetase activity, activation of the 2-5A-dependent RNase L could not be demonstrated in JCA-1 cells rendered permeable to 2-5A, implying that the 2-5A pathway is not involved in the anti-proliferative effects of IFN. Analysis of endogenous proteins phosphorylated in vitro show that some IFN-inducible phosphoproteins were dependent upon the presence of double-stranded DNA.
添加干扰素-β导致人前列腺JCA-1细胞的生长呈剂量依赖性降低，[3H]胸苷掺入DNA减少，同时S期和G2/M期分别增加69%和15%。干扰素的抗有丝分裂作用与双链RNA依赖性蛋白激酶活性的增加之间不存在相关性。尽管干扰素引起2-5A合成酶活性大幅增加，但在对2-5A通透的JCA-1细胞中未证实2-5A依赖性核糖核酸酶L的激活，这意味着2-5A途径不参与干扰素的抗增殖作用。对体外磷酸化的内源性蛋白质的分析表明，一些干扰素诱导的磷蛋白依赖于双链DNA的存在。
