Bertram J, Killian M, Brysch W, Schlingensiepen K H, Kneba M
Dept. of Hematology/Onc. Univ. Clinics, Göttingen, FRG.
Biochem Biophys Res Commun. 1994 Apr 15;200(1):661-7. doi: 10.1006/bbrc.1994.1499.
A considerable reduction of up to 75% at the protein level of the erbB2 gene product was observed using phosphorothioate antisense oligonucleotides directed against specific sequences of the erbB2 mRNA. Antisense oligonucleotides used were the 14-mer (translation start region) and the 17-mer (3' translated region) all-phosphorothioate oligonucleotides (S-ODNs). Sense or random sequences were used as a control. The greatest reduction at the erbB2 protein level was obtained after a 24h incubation with a single dose of 2 microM antisense S-ODN. The erbB2-mRNA-specific and the tyrosine kinase consensus antisense S-ODNs were comparably effective in inhibiting erbB2 expression. Addition of the transfection reagent DOTAP diminished the efficiency of erbB2 protein reduction by antisense S-ODNs after an incubation period of 24h but was more effective after 48h compared to the application of antisense S-ODNs alone.
使用针对erbB2 mRNA特定序列的硫代磷酸酯反义寡核苷酸，在蛋白质水平观察到erbB2基因产物显著降低多达75%。所使用的反义寡核苷酸为14聚体（翻译起始区域）和17聚体（3'翻译区域）全硫代磷酸酯寡核苷酸（S-ODN）。正义或随机序列用作对照。用单剂量2 microM反义S-ODN孵育24小时后，erbB2蛋白水平降低最为显著。erbB2-mRNA特异性和酪氨酸激酶共有序列反义S-ODN在抑制erbB2表达方面效果相当。转染试剂DOTAP的添加在孵育24小时后降低了反义S-ODN降低erbB2蛋白的效率，但与单独应用反义S-ODN相比，在48小时后更有效。
