Ambrosino D M, Wang M, Ciamarra A, Chan M, Bolon D L, Minn J, Jacobsohn D A, Finberg R W
Laboratory of Infectious Diseases, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
Cell Immunol. 1994 Apr 15;155(1):134-43. doi: 10.1006/cimm.1994.1107.
Cytokine regulation of human IgG subclass production is not well understood. Since T cells and natural killer (NK) cells produce IL-4 and/or IFN-gamma, we examined the effect of these cells on human IgG subclass concentration in reconstituted severe combined immunodeficient (SCID) mice. SCID mice receiving only B-cell-enriched splenocyte preparations had significantly decreased IgG concentrations and significantly decreased IgG1/IgG2 ratios compared to mice receiving B cells plus T cells (P = 0.02). IgG2 represented 58% of the total IgG at 28 days in mice receiving B-cell-enriched preparations compared to 19% of the total for the group receiving both B cells and T cells (P = 0.013). The effect of natural killer cells (CD16+) on IgG subclass was also studied in this model. IgG2 concentrations were twofold higher in mice receiving CD16-depleted cells compared to controls (P = 0.004). No significant differences were noted for IgG1, IgG3, or IgG4 subclass concentrations. In conclusion, T cells and natural killer cells influence human IgG subclass regulation in the SCID mouse model. We propose that the regulation of human IgG subclass production can be further examined in the SCID model.
细胞因子对人IgG亚类产生的调节作用尚未完全明确。由于T细胞和自然杀伤（NK）细胞可产生IL-4和/或γ干扰素，我们研究了这些细胞对重建的重症联合免疫缺陷（SCID）小鼠体内人IgG亚类浓度的影响。与接受B细胞加T细胞的小鼠相比，仅接受富含B细胞的脾细胞制剂的SCID小鼠的IgG浓度显著降低，IgG1/IgG2比率也显著降低（P = 0.02）。在接受富含B细胞制剂的小鼠中，IgG2在28天时占总IgG的58%，而在接受B细胞和T细胞的组中，该比例为19%（P = 0.013）。在该模型中还研究了自然杀伤细胞（CD16+）对IgG亚类的影响。与对照组相比，接受去除CD16细胞的小鼠的IgG2浓度高出两倍（P = 0.004）。IgG1、IgG3或IgG4亚类浓度未观察到显著差异。总之，在SCID小鼠模型中，T细胞和自然杀伤细胞影响人IgG亚类的调节。我们建议可在SCID模型中进一步研究人IgG亚类产生的调节。
