Greven H, Passia D, Marani E
Institut für Zoologie (Morphologie und Zellbiologie), Heinrich-Heine Universität, Düsseldorf, Germany.
Eur J Morphol. 1993 Dec;31(4):267-73.
The antigen CD15, also known as 3-fucosyl-N-acetyl-lactosamine (FAL), or Stage Specific Embryonic Antigen (SSEA-1) is a cell membrane and cytoplasmic antigen, that is present in several mammalian tissues. Using immunohistochemistry the monoclonal antibodies 3B9 and B4,3 detected CD15 in whole mount preparations of trophotaeniae of embryos of the matotroph viviparous teleost Xenotoca eiseni. A specific localization is encountered in the ecto- and entodermal portion of the trophotaenial epithelium. A positive reaction in the fibrocytes of the inner mesenchymal core of trophotaeniae is doubtful since a relative high background is present in these cells. CD15 positivity has been observed in all developmental stages studied. The exact role of CD15 is unclear. An involvement in endocytic activity is postulated.
抗原CD15，也称为3-岩藻糖基-N-乙酰乳糖胺（FAL）或阶段特异性胚胎抗原（SSEA-1），是一种存在于多种哺乳动物组织中的细胞膜和细胞质抗原。使用免疫组织化学方法，单克隆抗体3B9和B4,3在胎生胎生硬骨鱼Xenotoca eiseni胚胎的滋养带全层制剂中检测到了CD15。在滋养带上皮的外胚层和内胚层部分发现了特异性定位。滋养带内间充质核心的纤维细胞中的阳性反应值得怀疑，因为这些细胞中存在相对较高的背景。在所有研究的发育阶段都观察到了CD15阳性。CD15的确切作用尚不清楚。推测其参与内吞活动。
