甲硫氨酸脑啡肽在CD4+和CD8+脾T细胞上被氨肽酶N水解。
Methionine enkephalin is hydrolyzed by aminopeptidase N on CD4+ and CD8+ spleen T cells.
作者信息
Miller B C, Thiele D L, Hersh L B, Cottam G L
机构信息
Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas 75235-9038.
出版信息
Arch Biochem Biophys. 1994 May 15;311(1):174-9. doi: 10.1006/abbi.1994.1223.
Exogenous methionine enkephalin incubated with CD4+ or CD8+ T cells purified from murine spleen is metabolized primarily, if not exclusively, by aminopeptidase N (aminopeptidase M, EC 3.4.11.2), a membrane-anchored ectopeptidase. The enzyme activity is identified by its substrate specificity, sensitivity to inhibition by amastatin, and immunoreactivity with antibody to rat kidney aminopeptidase N. Activation of CD4+ T cells results in a small increase per cell in aminopeptidase N activity.
与从小鼠脾脏纯化的CD4⁺或CD8⁺T细胞一起孵育的外源性甲硫氨酸脑啡肽，主要（如果不是唯一的话）由氨肽酶N（氨肽酶M，EC 3.4.11.2）代谢，氨肽酶N是一种膜锚定的外肽酶。该酶活性通过其底物特异性、对氨肽素抑制的敏感性以及与抗大鼠肾氨肽酶N抗体的免疫反应性来鉴定。CD4⁺T细胞的激活导致每个细胞的氨肽酶N活性略有增加。
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